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porous polystyrene insert alvetex  (ReproCELL)

 
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    ReproCELL porous polystyrene insert alvetex
    Porous Polystyrene Insert Alvetex, supplied by ReproCELL, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/porous polystyrene insert alvetex/product/ReproCELL
    Average 94 stars, based on 6 article reviews
    porous polystyrene insert alvetex - by Bioz Stars, 2026-03
    94/100 stars

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    porous polystyrene insert alvetex  (ReproCELL)
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    Ageing dermal compartments exhibit decreased extracellular matrix deposition. Neonatal or ageing fibroblasts were seeded in <t>Alvetex®</t> Scaffold and cultured for 30 days before being harvested for analysis (a). H&E staining of dermal compartments generated with neonatal (bi) and ageing fibroblasts (bii) demonstrate fibroblast population of the scaffold. Representative immunofluorescence staining of collagen I (biii) and collagen III (biv) is reduced in ageing dermal compartments. Collagens are stained green and nuclei are stained with DAPI. Scale bars: B = 50 μm.
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    Ageing dermal compartments exhibit decreased extracellular matrix deposition. Neonatal or ageing fibroblasts were seeded in <t>Alvetex®</t> Scaffold and cultured for 30 days before being harvested for analysis (a). H&E staining of dermal compartments generated with neonatal (bi) and ageing fibroblasts (bii) demonstrate fibroblast population of the scaffold. Representative immunofluorescence staining of collagen I (biii) and collagen III (biv) is reduced in ageing dermal compartments. Collagens are stained green and nuclei are stained with DAPI. Scale bars: B = 50 μm.
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    Ageing dermal compartments exhibit decreased extracellular matrix deposition. Neonatal or ageing fibroblasts were seeded in <t>Alvetex®</t> Scaffold and cultured for 30 days before being harvested for analysis (a). H&E staining of dermal compartments generated with neonatal (bi) and ageing fibroblasts (bii) demonstrate fibroblast population of the scaffold. Representative immunofluorescence staining of collagen I (biii) and collagen III (biv) is reduced in ageing dermal compartments. Collagens are stained green and nuclei are stained with DAPI. Scale bars: B = 50 μm.
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    Ageing dermal compartments exhibit decreased extracellular matrix deposition. Neonatal or ageing fibroblasts were seeded in <t>Alvetex®</t> Scaffold and cultured for 30 days before being harvested for analysis (a). H&E staining of dermal compartments generated with neonatal (bi) and ageing fibroblasts (bii) demonstrate fibroblast population of the scaffold. Representative immunofluorescence staining of collagen I (biii) and collagen III (biv) is reduced in ageing dermal compartments. Collagens are stained green and nuclei are stained with DAPI. Scale bars: B = 50 μm.
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    Ageing dermal compartments exhibit decreased extracellular matrix deposition. Neonatal or ageing fibroblasts were seeded in <t>Alvetex®</t> Scaffold and cultured for 30 days before being harvested for analysis (a). H&E staining of dermal compartments generated with neonatal (bi) and ageing fibroblasts (bii) demonstrate fibroblast population of the scaffold. Representative immunofluorescence staining of collagen I (biii) and collagen III (biv) is reduced in ageing dermal compartments. Collagens are stained green and nuclei are stained with DAPI. Scale bars: B = 50 μm.
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    alvetex ® scaffold inserts  (ReproCELL)
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    Development and characterisation of in vitro IBD mucosal constructs. (A) Workflow schematic for model set-up using <t>Alvetex</t> ® Scaffold. (B) H&E image of unstimulated IBD model and IBD human tissue (HT) for comparison. Scale bars 50 μm. (C) TEM micrographs of epithelial polarisation (C ,a ) , and microvilli (m) brush border with clear rootlet (r) visibility (C ,b ) , apical-junctional complexes consisting of tight junction (tj), adherens junction (aj) and desmosome (dm) (C ,c ) , basement membrane (bm) formation with vesicle exocytosis (ve) (C ,d ) , and the lamina propria composition containing fibroblasts (f) and longitudinal collagen and transverse collagen (lc and tc) (C ,e ) . Scale bars: 10 μm (C ,a ) , 1 μm ( C ,b + C ,d), 0.5 μm (C ,c ) and 5 μm (C ,e ) . (D) Representative IF images of fibroblast markers ( D ,a + D ,b), pan-macrophage marker CD68 (D ,c ) and ECM protein expression ( D ,d, D ,e + D ,f) within Alvetex ® lamina propria-like compartment. Scale bars 50 μm. (E) IF staining of epithelial and polarisation markers in IBD model epithelial tissue. Scale bars 50 μm.
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    Image Search Results


    Ageing dermal compartments exhibit decreased extracellular matrix deposition. Neonatal or ageing fibroblasts were seeded in Alvetex® Scaffold and cultured for 30 days before being harvested for analysis (a). H&E staining of dermal compartments generated with neonatal (bi) and ageing fibroblasts (bii) demonstrate fibroblast population of the scaffold. Representative immunofluorescence staining of collagen I (biii) and collagen III (biv) is reduced in ageing dermal compartments. Collagens are stained green and nuclei are stained with DAPI. Scale bars: B = 50 μm.

    Journal: Journal of Cellular Physiology

    Article Title: Investigation into the significant role of dermal‐epidermal interactions in skin ageing utilising a bioengineered skin construct

    doi: 10.1002/jcp.31463

    Figure Lengend Snippet: Ageing dermal compartments exhibit decreased extracellular matrix deposition. Neonatal or ageing fibroblasts were seeded in Alvetex® Scaffold and cultured for 30 days before being harvested for analysis (a). H&E staining of dermal compartments generated with neonatal (bi) and ageing fibroblasts (bii) demonstrate fibroblast population of the scaffold. Representative immunofluorescence staining of collagen I (biii) and collagen III (biv) is reduced in ageing dermal compartments. Collagens are stained green and nuclei are stained with DAPI. Scale bars: B = 50 μm.

    Article Snippet: Briefly, 0.5 × 10 6 HDFn or HDFa were seeded into 12 well Alvetex® Scaffold inserts (Reprocell Europe Ltd, Glasgow, UK), a porous, inert, polystyrene scaffold.

    Techniques: Cell Culture, Staining, Generated, Immunofluorescence

    Development and characterisation of in vitro IBD mucosal constructs. (A) Workflow schematic for model set-up using Alvetex ® Scaffold. (B) H&E image of unstimulated IBD model and IBD human tissue (HT) for comparison. Scale bars 50 μm. (C) TEM micrographs of epithelial polarisation (C ,a ) , and microvilli (m) brush border with clear rootlet (r) visibility (C ,b ) , apical-junctional complexes consisting of tight junction (tj), adherens junction (aj) and desmosome (dm) (C ,c ) , basement membrane (bm) formation with vesicle exocytosis (ve) (C ,d ) , and the lamina propria composition containing fibroblasts (f) and longitudinal collagen and transverse collagen (lc and tc) (C ,e ) . Scale bars: 10 μm (C ,a ) , 1 μm ( C ,b + C ,d), 0.5 μm (C ,c ) and 5 μm (C ,e ) . (D) Representative IF images of fibroblast markers ( D ,a + D ,b), pan-macrophage marker CD68 (D ,c ) and ECM protein expression ( D ,d, D ,e + D ,f) within Alvetex ® lamina propria-like compartment. Scale bars 50 μm. (E) IF staining of epithelial and polarisation markers in IBD model epithelial tissue. Scale bars 50 μm.

    Journal: Frontiers in Immunology

    Article Title: An in vitro model to study immune activation, epithelial disruption and stromal remodelling in inflammatory bowel disease and fistulising Crohn’s disease

    doi: 10.3389/fimmu.2024.1357690

    Figure Lengend Snippet: Development and characterisation of in vitro IBD mucosal constructs. (A) Workflow schematic for model set-up using Alvetex ® Scaffold. (B) H&E image of unstimulated IBD model and IBD human tissue (HT) for comparison. Scale bars 50 μm. (C) TEM micrographs of epithelial polarisation (C ,a ) , and microvilli (m) brush border with clear rootlet (r) visibility (C ,b ) , apical-junctional complexes consisting of tight junction (tj), adherens junction (aj) and desmosome (dm) (C ,c ) , basement membrane (bm) formation with vesicle exocytosis (ve) (C ,d ) , and the lamina propria composition containing fibroblasts (f) and longitudinal collagen and transverse collagen (lc and tc) (C ,e ) . Scale bars: 10 μm (C ,a ) , 1 μm ( C ,b + C ,d), 0.5 μm (C ,c ) and 5 μm (C ,e ) . (D) Representative IF images of fibroblast markers ( D ,a + D ,b), pan-macrophage marker CD68 (D ,c ) and ECM protein expression ( D ,d, D ,e + D ,f) within Alvetex ® lamina propria-like compartment. Scale bars 50 μm. (E) IF staining of epithelial and polarisation markers in IBD model epithelial tissue. Scale bars 50 μm.

    Article Snippet: In vitro IBD mucosal constructs were generated using Alvetex ® Scaffold inserts (Reprocell Europe Ltd, UK).

    Techniques: In Vitro, Construct, Comparison, Membrane, Marker, Expressing, Staining